Authors: Li Panpan, Guan Huaijin
Corresponding author: Guan Huaijin, Email: guanhuaijinleye@126.com
Published:2026-07-10
DOI: 10.3760/cma.j.cn115989-20231124-00184
ABSTRACT
The clinical diagnosis of infectious diseases mainly relies on the isolation and culture of pathogenic microorganisms, but many infectious eye diseases have problems such as acute onset, difficulty in obtaining samples, and difficulty in diagnosis. Compared with traditional microbial culture, molecular diagnostic techniques (MDT) can quickly realize the identification and quantitative detection of pathogenic microorganisms, providing an accurate diagnosis basis for clinical practice. PCR based on nucleic acid amplification technology takes a short time and is highly sensitive, which greatly improves the detection rate of pathogenic microorganisms. However, the polymerase chain reaction can only detect one or several pathogens at a time and can only be used for the detection of known pathogenic microorganisms. Gene sequencing not only ensures high sensitivity but also has the advantages of a wide detection range and high throughput. Metagenomics sequencing can discover new pathogens, but gene sequencing is expensive. This paper summarizes and analyzes the application of MDT based on gene amplification technology, gene sequencing technology, nucleic acid molecular hybridization technology and gene chip technology in the diagnosis of infectious ophthalmic diseases, in order to provide a reference for clinical diagnosis work.
KEYWORDS：
Molecular diagnostic techniques;Infectious eye disease;Keratitis;Endophthalmitis;Infectious uveitis
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Authors Info & Affiliations
Li Panpan
Department of Ophthalmology, Nantong First People’s Hospital, Nantong 226001, China
Guan Huaijin
Department of Ophthalmology, Affiliated Hospital of Nantong University, Nantong 226001, China
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