Expression and significance of nuclear factor-κB-related proteins in idiopathic orbital inflammatory pseudotumor

Authors: Yang Linqi,  Zhao Pengxiang,  Wu Yanan,  Zhang Xujuan,  Shang Lei,  Liu Mengyu,  Liu Xiao,  Ma Jianmin,  Ma Xuemei

DOI: 10.3760/cma.j.issn.2095-0160.2017.09.004
Published 2017-09-10
Cite as Chin J Exp Ophthalmol, 2017,35(9): 786-791.

Abstract

Background

Idiopathic orbital inflammatory pseudotumor (IOIP) is a commom orbital disease, with serious eye symptoms and replase tendency, and its pathogenesis is still unclear.Nuclear factor-κB (NF-κB)-related proteins participate in many important pathophysiological process, however, whether NF-κB plays a role in the IOIP process is worthy of attention.

Objective

This study was to explore the roles of NF-κB pathway in IOIP pathogenesis.

Methods

Twenty-four IOIP specimens were collected during surgery in Beijing Tongren Hospital from September 2010 to May 2016.The histopathological characteristics of IOIP were examined by hematoxylin and eosin staining.The expression and location of NF-κB/p65, p-p65, p50 and inhibitor of κB (IκB-α) were detected by immunohistochemistry and verified by immunocytochemistry and Western blot assay.

Results

The histopathological features of IOIP were numerous small lymphocyte infiltraion and fibrous tissue proliferation, and a lot of epithelioid cells were seen in lacrimal gland-involved specimens.NF-κB/p65 was positively expressed in the cytoplasm of all 24 specimens and the nucleus in 15 specimens with the expressing rate of 62.5%.p50 was expressed in the cytoplasm in 22 specimens with the expressing rate of 91.7% and in the nucleus in 17 specimens with the expressing rate of 70.8%.The positive expression of p-p65 was found in 22 specimens with the expressing rate of 91.7%, and IκB-α was expressed in the cytoplasm of 11 specimens with the expressing rate of 45.8%.These results were confirmed by immunocytochemistry and Western blot assay.

Conclusions

NF-κB pathway is activiated during IOIP process, and NF-κB pathway may be involved in the pathogenesis of IOIP.

Key words:

Nuclear factor-kappa B/metabolism; Orbital inflammatory pseudotumor/pathology; Inflammation; Immunochemistry; Humans; Nuclear proteins/metabolism; Signal transduction pathway

Contributor Information

Yang Linqi
College of Life Science and Bioengineering of Beijing University of Technology, Beijing 100124, China
Zhao Pengxiang
College of Life Science and Bioengineering of Beijing University of Technology, Beijing 100124, China
Wu Yanan
College of Life Science and Bioengineering of Beijing University of Technology, Beijing 100124, China
Zhang Xujuan
College of Life Science and Bioengineering of Beijing University of Technology, Beijing 100124, China
Shang Lei
College of Life Science and Bioengineering of Beijing University of Technology, Beijing 100124, China
Liu Mengyu
College of Life Science and Bioengineering of Beijing University of Technology, Beijing 100124, China
Liu Xiao
Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Science, Beijing 100730, China
Ma Jianmin
Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Science, Beijing 100730, China
Ma Xuemei
College of Life Science and Bioengineering of Beijing University of Technology, Beijing 100124, China
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