Category: Experimental Science

Quantitative proteomic analysis of human umbilical cord mesenchymal stem cells with pigment epithelium-derived factor gene modification mediated by lentivirus

Authors: Jiang Yilin,  Zhang Xiaomin,  Shao Xianfeng,  An Jinying,  Su Lin,  Jiang Yuanfeng,  Li Xiaorong DOI: 10.3760/cma.j.cn115989-20200609-00408 Published 2021-10-10 Cite asChin J Exp Ophthalmol, 2021, 39(10): 845-851. Abstract Objective To investigate the protein expression changes of human umbilical cord mesenchymal stem cells (hUCMSCs) modified with pigment epithelium-derived factor (PEDF) gene mediated by lentivirus. Methods The […]

Breakdown of blood-retinal outer barrier in mice following acute high intraocular pressure

Authors: Li Wen,  Cao Xing,  Ye Changhua DOI: 10.3760/cma.j.cn115989-20200305-00146 Published 2021-10-10 Cite asChin J Exp Ophthalmol, 2021, 39(10): 852-856. Abstract Objective To investigate the breakdown of blood-retinal outer barrier in the ischemia-reperfusion injury mice following acute intraocular hypertension. Methods Fifty-seven SPF male C57BL/6J mice were selected and divided into the control group and high-intraocular pressure […]

Effect of long-term topical administration of prostaglandins on bulbar conjunctival thickness in rabbits

Authors: Ma Kai,  Yin Chengjuan,  Zhang Zhenyong DOI: 10.3760/cma.j.cn115989-20200707-00481 Published 2021-10-10 Cite asChin J Exp Ophthalmol, 2021, 39(10): 857-862. Abstract Objective To investigate the effect of long-term application of prostaglandin analog drops on bulbar conjunctival thickness in rabbits. Methods Twenty-four healthy New Zealand white rabbits were randomly divided into latanoprost group, carteolol group and blank […]

Studies on the mechanism of inhibitory effect of lithium chloride on the proliferation of human Tenon capsule fibroblasts

Authors: Zou Huihui,  Liu Shanshan,  Liang Ling,  Fan Xiaojun,  Wang Jibing DOI: 10.3760/cma.j.cn115989-20200324-00196 Published 2021-10-10 Cite asChin J Exp Ophthalmol, 2021, 39(10): 863-868. Abstract Objective To investigate the effect of lithium chloride (LiCl) on the gap junctional intercellular communication (GJIC) in human Tenon capsule fibroblasts (HTFs) and its underlying mechanism. Methods The Tenon capsule tissue […]

Evaluation of low-quality fundus image enhancement based on cycle-constraint adversarial network

Authors: Zhou Xueting,  Yang Weihua,  Hua Xiao,  You Qijing,  Sun Jing,  Shen Jianxin,  Wan Cheng DOI: 10.3760/cma.j.cn115989-20201028-00718 Published 2021-09-10 Cite asChin J Exp Ophthalmol, 2021, 39(9): 769-775. Abstract Objective To propose and evaluate the cycle-constraint adversarial network (CycleGAN) for enhancing the low-quality fundus images such as the blurred, underexposed and overexposed etc. Methods A dataset […]

Regulatory effect of edaravone on the photoreceptor autophagy at the early stage of experimental retinal detachment in rats

Authors: Kang Ziwei,  Ren Xiuyu,  Zhang Yanchun,  Pei Cheng,  Zhang Nana,  Xia Yimin,  Yang Jingru DOI: 10.3760/cma.j.cn115989-20200228-00123 Published 2021-09-10 Cite asChin J Exp Ophthalmol, 2021, 39(9): 776-784. Abstract Objective To investigate the effect of edaravone, a free radical scavenger, on the regulation of retinal autophagy and the protection of photoreceptor cells at the early stage […]

Evaluation of multi-classification method of color fundus photograph quality based on ResNet50-OC

Authors: Wan Cheng,  Zhou Xueting,  You Qijing,  Shen Jianxin,  Yu Qiuli DOI: 10.3760/cma.j.cn115989-20200107-00011 Published 2021-09-10 Cite asChin J Exp Ophthalmol, 2021, 39(9): 785-790. Abstract Objective To evaluate the efficiency of ResNet50-OC model based on deep learning for multiple classification of color fundus photographs. Methods The proprietary dataset (PD) collected in July 2018 in BenQ Hospital […]

Expression of lncRNA Tsix in a mouse model of N-methyl-D-aspartic acid-induced retinal excitotoxicity and its significance

Authors: Li Yahong,  Geng Chao,  Hei Kaiwen,  Liu Shengnan,  Wang Qi,  Li Xiaorong,  Zhang Yan DOI: 10.3760/cma.j.cn115989-20200119-00033 Published 2021-08-10 Cite asChin J Exp Ophthalmol, 2021, 39(8): 676-685. Abstract Objective To investigate the damage effect of different concentrations of N-methyl-D-aspartic acid (NMDA) to retinal ganglion cells (RGCs) in mice and explore the expression of long noncoding […]

Bioinformatics analysis of transcriptome sequencing after the interference of extremely low frequency electromagnetic field in mouse fibroblasts

Authors: Zhu Maolin,  Zhu Huang DOI: 10.3760/cma.j.cn115989-20200905-00633 Published 2021-08-10 Cite asChin J Exp Ophthalmol, 2021, 39(8): 686-692. Abstract Objective To analyze the changes in the transcriptome of mouse fibroblasts after exposure to extremely low frequency electromagnetic fields (ELF-EMFs) using next-generation sequencing technology, and to screen out related pathways and genes that might be involved in […]

Protective effect of adeno-associated virus vector mediated heme oxygenase-1 overexpression on retinitis pigmentosa in rats

Authors: Liang Yuping,  Chen Weiqi,  Hong Yu,  Gao Xiuyun,  Guo Maosheng DOI: 10.3760/cma.j.cn115989-20201108-00754 Published 2021-08-10 Cite asChin J Exp Ophthalmol, 2021, 39(8): 693-699. Abstract Objective To investigate the protective effect of adeno-associated virus (AAV) vector-mediated heme oxygenase-1 (HO-1) gene overexpression on retinitis pigmentosa (RP) models in rats. Methods Eighty healthy male SD rats were selected […]