Category: Experimental Science

Inhibitory effect and biological mechanism of Dickkopf-1 on the proliferation of human lens epithelial cells

Authors: Zhang Limin,  Bao Xiuli DOI: 10.3760/cma.j.cn.115985-20190328-00151 Published 2020-04-10 Cite as Chin J Exp Ophthalmol, 2020,38(04): 285-290. Abstract Objective To investigate the effects of Dickkopf-1(DKK1) on the proliferation of human lens epithelial cells (LECs) and its possible mechanism in order to search a new target for the treatment of posterior capsular opacification (PCO). Methods Human LECs […]

Changes in DNA methylation of retinal gene promoter regions and their significance in type 1 diabetic rats

Authors: Li Yahong,  Geng Chao,  Liu Shengnan,  Wang Qi,  Li Xiaorong,  Zhang Yan DOI: 10.3760/cma.j.cn115985-20190616-00262 Published 2020-04-10 Cite as Chin J Exp Ophthalmol, 2020,38(04): 291-299. Abstract Objective To study the methylation level of retinal gene promoter region in a rat model of type 1 diabetes (T1D) and investigate the correlation between DNA methylation level and retinal […]

Effects of femtosecond laser mushroom-like penetrating ring-cut on ring incision and corneal endothelial cells

Authors: Jia Guizhen,  Shi Yunqing,  Cheng Mengya,  Cao Xueqian,  Wang Linnong DOI: 10.3760/cma.j.cn115985-20200325-00200 Published 2020-04-10 Cite as Chin J Exp Ophthalmol, 2020,38(04): 300-304. Abstract Objective To evaluate the effect of mushroom-like pattern femtosecond laser enabled penetrating keratoplasty with different laser energies on the ring incision and corneal endothelial cells, and compare with conventional penetrating keratoplasty. Methods According to the point/line separation […]

In vitroantimicrobial activity of diacerein on common ocular surface pathogens

Authors:Yue Juan,  Jian Shoujun,  Lyu Qixue,  Sun Shengtao,  Han Lei,  Liu Susu,  Zhang Hongmin DOI: 10.3760/cma.j.issn.2095-0160.2020.03.002 Published 2020-03-10 Cite as Chin J Exp Ophthalmol, 2020,38(03): 165-168. Abstract Objective To study the antimicrobial activity of diacerein on common pathogens of the ocular surface in vitro. Methods Pathogens were collected from patients with ocular surface infections in Henan […]

Efficacy and safety of modified chitosan eye drops in the treatment of Candida albicanskeratitis in rabbits

Authors:Yang Ruibo,  Gao Yichen,  Zhu Meng,  Niu Zhongwei,  Zhang Chen,  Liu Hui,  Huang Yue,  Gao Yunhua,  Zhao Shaozhen DOI: 10.3760/cma.j.issn.2095-0160.2020.03.003 Published 2020-03-10 Cite as Chin J Exp Ophthalmol, 2020,38(03): 169-174. Abstract Objective To evaluate the clinical efficacy and safety of modified chitosan eye drops on rabbit Candida albicans keratitis model. Methods Ten healthy female New Zealand […]

Different contact lens case treatment methods for the elimination of pathogen biofilms associated with contact lens related microbial keratitis

Authors: Wang Shuai,  Wang Qiangyi,  Li Yutang,  Deng Juan,  Su Mingze,  He Lingyuan,  Wang Luwei,  Li Tong DOI: 10.3760/cma.j.issn.2095-0160.2020.03.004 Published 2020-03-10 Cite as Chin J Exp Ophthalmol, 2020,38(03): 175-180. Abstract Objective To investigate the biofilm-forming abilities of pathogens associated with contact lens related microbial keratitis and to compare the efficacies of different treatments in eliminating biofilms […]

In vivo investigation of the effectiveness of reactive oxygen species-responsive nanomedicine in suppressing corneal neovascularization

Authors: Liu Anqi,  Liang Chunjing,  Wang Ming,  Wang Liqiang,  Huang Yifei DOI: 10.3760/cma.j.issn.2095-0160.2020.02.002 Published 2020-02-10 Cite as Chin J Exp Ophthalmol, 2020,38(02): 85-92. Abstract Objective To evaluate the effectiveness of reactive oxygen species (ROS)-responsive nanomedicine in suppressing corneal neovascularization (CNV) in vivo. Methods ROS-responsive nanomedicine (ROS-TK-5/siVEGF), which consists of vascular endothelial growth factor (VEGF) small interfering RNA […]

Procedure for early corneal basement membrane repair and regeneration in corneal penetrating injury in rabbits

Authors: Xu Luxing,  Wu Jinling,  Wang Shuangning,  Li Xia DOI: 10.3760/cma.j.issn.2095-0160.2020.02.003 Published 2020-02-10 Cite as Chin J Exp Ophthalmol, 2020,38(02): 93-99. Abstract Objective To describe the procedure for early corneal epithelial basement membrane(EBM) repair and regeneration in rabbits with corneal penetrating injury. Methods Forty-two New Zealand white rabbits were divided into modeling 1-, 3-, 5-, 7-, […]

Inhibitory effects of NADPH oxidase 4 inhibitor on VEGF expression in hypoxia-induced human RPE cells

Authors: Li Jing,  Yang Jing,  Kang Qianyan DOI: 10.3760/cma.j.issn.2095-0160.2020.01.002 Published 2020-01-10 Cite as Chin J Exp Ophthalmol, 2020,38(01): 4-9. Abstract Objective To observe the inhibitory effects of nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) on vascular endothelial growth factor (VEGF) expression in hypoxia-induced human retinal pigment epithelial cells. Methods The cultured APRE-19 cells were divided into […]

Damage of blue-light exposure to retinal morphology and function in mice

Authors: Yang Daidi,  Sun Ruxu,  Chen Xue,  Liu Qinghuai DOI: 10.3760/cma.j.issn.2095-0160.2020.01.003 Published 2020-01-10 Cite as Chin J Exp Ophthalmol, 2020,38(01): 10-15. Abstract Objective To study the damage effects of blue-light exposure on retinal morphology and function in mouse. Methods Twenty 8-week-old clean C57BL/6J mice were randomly divided into blue-light exposure group and normal control group by […]