Category: Experimental Science

Protective effect and mechanism of cx3cr1 antibody intravitreal injection in microcirculation of retinal ischemia-reperfusion injury in mice

Authors: Li Juanjuan,  Chen Chen,  Li Yan,  Zhang Liwei DOI: 10.3760/cma.j.cn115989-20200104-00004 Published 2021-07-10 Cite asChin J Exp Ophthalmol, 2021, 39(7): 585-592. Abstract Objective To investigate the protective effect of C-X3-C motif chemokine receptor 1 (cx3cr1) antibody, a microglia activation inhibitor, on microcirculation during retinal ischemia reperfusion (RIR) and its possible mechanism. Methods One hundred and […]

Protective effect of asiatic acid on blood-retinal barrier in diabetic rats

Authors: Fang Mengyuan,  Li Qiuming,  Yang Xin,  Wan Weiwei,  Long Yang,  Liu Hongzhuo,  Wan Wencui DOI: 10.3760/cma.j.cn115989-20201024-00713 Published 2021-07-10 Cite asChin J Exp Ophthalmol, 2021, 39(7): 593-601. Abstract Objective To investigate the protective effect of asiatic acid (AA) on blood-retinal barrier (BRB) in diabetic rats and its possible mechanism. Methods Ninety-six healthy 8-week-old male SD […]

Research on screening system of myopic maculopathy based on deep convolution neural network

Authors: Shi Chunsheng,  Liu Lei,  Wang Yaru,  Wang Zefei DOI: 10.3760/cma.j.cn115989-20191115-00495 Published 2021-07-10 Cite asChin J Exp Ophthalmol, 2021, 39(7): 602-608. Abstract Objective To develop a fully automatic detection system based on the deep convolution neural network (DCNN) for screening myopic maculopathy (MMD) and identifying its severity. Methods Six thousand and sixty-eight fundus images were […]

Inhibiting effect of retinal Sigma-1 receptor antagonist on the form deprivation myopia in guinea pigs and its mechanism

Authors: Chen Yuanyuan,  Xie Fujuan,  Li Haibo,  Guan Yuxin,  Mao Junfeng DOI: 10.3760/cma.j.cn115989-20200615-00433 Published 2021-06-10 Cite asChin J Exp Ophthalmol, 2021, 39(6): 477-482. Abstract Objective To investigate the role and mechanism of retinal Sigma-1 receptor antagonist N, N-diethyl-2-[4-methoxy-3-(2-phenylethoxy)phenyl]ethanaminehydrochloride (NE-100) in the formation of form deprivation myopia (FDM) in guinea pigs. Methods Eighty-five 21-day-old guinea pigs […]

Relationship between irradiation time of riboflavin-ultraviolet A scleral collagen cross-linking and retinal injury

Authors: Yan Dong,  Liu Limei,  Ming Chunxiu,  Zhang Shaobin DOI: 10.3760/cma.j.cn115989-20190703-00287 Published 2021-06-10 Cite asChin J Exp Ophthalmol, 2021, 39(6): 483-489. Abstract Objective To investigate the effects of riboflavin-ultraviolet A scleral collagen cross-linking on the retina under different irradiation time, and to determine the safe irradiation time. Methods Sixty healthy New Zealand white rabbits were […]

All-trans retinoic acid-induced apoptosis signaling pathway in ARPE-19 cells

Authors: Wu Juan,  Cui Dongmei,  Yang Xiao,  Zeng Junwen DOI: 10.3760/cma.j.cn115989-20190704-00293 Published 2021-06-10 Cite asChin J Exp Ophthalmol, 2021, 39(6): 490-497. Abstract Objective To investigate all-trans retinoic acid(ATRA)-induced apoptosis signaling pathway in ARPE-19 cells in vitro. Methods The APRE-19 cell was treated with different concentrations of ATRA for 24 hours and 48 hours.Cell counting kit-8 […]

Label-free quantitative proteomic analysis of aqueous humor in patients with high myopia

Authors: Xue Min,  Ren Xinjun,  Ke Yifeng,  Liu Juping,  Fan Xiaoe,  Li Xiaorong DOI: 10.3760/cma.j.cn115989-20200716-00503 Published 2021-06-10 Cite asChin J Exp Ophthalmol, 2021, 39(6): 498-504. Abstract Objective To characterize proteomic profile in aqueous humor of patients with high myopia using quantitative proteomic analysis. Methods Sixty-eight age-related cataract patients were divided into high myopic cataract group […]

Transcriptomics analysis of hypoxia-induced retinal pigment epithelium cell injury

Authors: Lu Cong,  Shi Pingling,  Yang Qixiang,  Song Hao,  Li Miao,  Zhang Beibei,  Song Zongming DOI: 10.3760/cma.j.cn115989-20201127-00799 Published 2021-06-10 Cite asChin J Exp Ophthalmol, 2021, 39(6): 505-514. Abstract Objective To analyze differentially expressed genes (DEGs) and the changes of signal pathways in human retinal pigment epithelium cells (ARPE-19) under hypoxic and normoxic conditions and to […]

Proteomics analysis of mouse lens supernatant treated with hydrogen peroxide in vitro

Authors: Li Guo,  Chen Ying,  Yan Hong,  Zhou Xiyuan DOI: 10.3760/cma.j.cn115989-20200730-00546 Published 2021-05-10 Cite asChin J Exp Ophthalmol, 2021, 39(5): 382-387. Abstract Objective To study the protein leakage of mouse lens treated with hydrogen peroxide in vitro, and to identify and carry out bioinformatics analysis of the leaked proteins. Methods Intact binocular lenses of 42 […]

Construction of Crx-iCreERT2 fluorescent reporter human embryonic stem cells by CRISPR/Cas9 technology and 3D retinal organoid culture

Authors: Du Yuxin,  Liu Yizong,  Yan Feiyue,  Shen Yin DOI: 10.3760/cma.j.cn115989-20210208-00103 Published 2021-05-10 Cite asChin J Exp Ophthalmol, 2021, 39(5): 388-397. Abstract Objective To establish Crx-iCreERT2 fluorescent reporter human embryonic stem cell lines using CRISPR/Cas9 technology and 3D retinal organoid culture. Methods The target site sequence of H9 cell line was verified by polymerase chain […]