Category: Experimental Science

Inhibitory effects of miR-146a on retinal inflammation induced by high glucose in human retinal endothelial cells

Authors: Gu Shun,  Zhan Pengfei,  Wang Wenjuan,  Wang Xiaolu,  Wei Tingting,  Zhu Lingpeng,  Wang Yangningzhi,  Yin Li,  Xie Tianhua,  Yao Yong DOI: 10.3760/cma.j.cn115989-20190213-00051 Published 2020-09-10 Cite as Chin J Exp Ophthalmol, 2020,38(09): 733-739. Abstract Objective To observe the effects of miR-146a on human retinal endothelial cell (HREC) under high glucose condition. Methods Total of 57 […]

Effect of recombinant human vascular endostatin on retinal barrier related proteins in early diabetic rats

Authors: Zhou Jinhua,  Xie Zhenggao DOI: 10.3760/cma.j.cn115989-20190228-00092 Published 2020-09-10 Cite as Chin J Exp Ophthalmol, 2020,38(09): 740-745. Abstract Objective To observe the effect of recombinant human vascular endostatin(ES) on retinal barrier related proteins in early streptozotocin (STZ)-induced diabetic rats. Methods Diabetes rat model was induced by STZ.Two weeks after the model was successfully constructed, 36 […]

Application of virus tracing tools in the study of function and morphology of neurons in the visual circuit

Authors: Shen Kejiong,  Shen Yin DOI: 10.3760/cma.j.cn115989-20191021-00457 Published 2020-09-10 Cite as Chin J Exp Ophthalmol, 2020,38(09): 746-753. Abstract Objective To utilize four viral tracer tools with different properties, to visualize cell morphology and connectome in visual pathways and explore and summarize the applications of different viral tracer tools in visual circuit, basing on the technology […]

Effects of miR-338-3p/TCF4 on choroidal microvascular endothelial cell proliferation and apoptosis

Authors: Li Jiancheng,  Bu Zhanyun,  Pan Junhui DOI: 10.3760/cma.j.cn115989-20200407-00242 Published 2020-08-10 Cite as Chin J Exp Ophthalmol, 2020,38(08): 639-645. Abstract Objective To investigate whether microRNA-338-3p (miR-338-3p) affects the proliferation and apoptosis of choroidal microvascular endothelial cells by regulating the expression of T cell factor 4 (TCF4). Methods Human choroidal microvascular endothelial cells were cultured in vitro, and […]

Differential gene expression profiles of trabecular meshwork between POAG and non-POAG donated eyes by using RNA-sequencing

Authors: Liu Lifang,  Zeng Jinhui,  Huang Chukai,  Wang Geng,  Zhang Mingzhi DOI: 10.3760/cma.j.cn115989-20191018-00451 Published 2020-08-10 Cite as Chin J Exp Ophthalmol, 2020,38(08): 646-652. Abstract Objective To investigate gene basis of primary open angle glaucoma (POAG) by comparing gene expression profile of trabecular meshwork between POAG patients and normal controls by using RNA-sequencing. Methods Trabecular meshwork specimen […]

In vitro interactions between antifungals and tacrolimus against Fusarium solani

Authors: Chen Xiaolian,  Ding Hui,  Liu Hongshan,  He Hong,  Wu Jiaochan,  Hu Xianlyu,  Zhong Xingwu DOI: 10.3760/cma.j.cn115989-20190613-00260 Published 2020-08-10 Cite as Chin J Exp Ophthalmol, 2020,38(08): 653-658. Abstract Objective To investigate the in vitro interaction between antifungals and tacrolimus against Fusarium solani. Methods According to Clinical and Laboratory Standards Institute(CLSI) M27-Ed4 and M38-A3, 24 strains of Fusarium solani isolated from clinic […]

Establishment and evaluation of a novel retinal ischemia-reperfusion injury model

Authors: Wen Xin,  Yuan Miner,  Li Cheng,  Zeng Jieting,  Lin Xiaofeng DOI: 10.3760/cma.j.cn115989-20190404-00162 Published 2020-07-10 Cite as Chin J Exp Ophthalmol, 2020,38(07): 566-572. Abstract Objective To explore the feasibility to establish a novel retinal ischemia reperfusion (RIR) injury model by applying pars plana vitrectomy (PPV) combined with retinal vascular counterpulsation in the central retinal artery occlusion […]

Safe dose of 650 nm low-power semiconductor laser irradiation in chicken retina

Authors: Wang Yipeng,  Chen Song,  Yang Wenchao,  Kong Jiahui DOI: 10.3760/cma.j.cn115989-20200519-00359 Published 2020-07-10 Cite as Chin J Exp Ophthalmol, 2020,38(07): 573-580. Abstract Objective To observe whether there was a chronic light damage after the irradiation of 650 nm semiconductor laser (power 2 mW) in chicken cone-rich-retina and discuss the safety of this laser for retina. Methods […]

Inhibiting effect of subconjuctivally injected conbercept on experimental corneal neovascularization and lymphangiogenesis in rabbit

Authors: Chen Xiaoxia,  Li Jing,  Gu Zhencheng DOI: 10.3760/cma.j.cn115989-20190327-00149 Published 2020-07-10 Cite as Chin J Exp Ophthalmol, 2020,38(07): 581-588. Abstract Objective To investigate the value of conbercept in experimental corneal nevascularization (CNV) and lymphangiogenesis in rabbit model. Methods Forty-four adult New Zealand rabbits were randomly divided into a conbercept injected group (9 rabbits), a ranibizumab injected […]

Protective effect of astaxanthin on the retinopathy of type 1 diabetic rats

Authors: Yang Ming,  Zhao Tong,  Deng Tingting,  Pan Lin,  Wang Zhijun DOI: 10.3760/cma.j.cn115989-20200519-00360 Published 2020-07-10 Cite as Chin J Exp Ophthalmol, 2020,38(07): 589-596. Abstract Objective To investigate the protective effect of astaxanthin on the retinopathy in rats with type 1 diabetes and related mechanism. Methods Thirty-six male SPF rats received intraperitoneal injection of 1% streptozotocin (STZ) […]