Category: Experimental Science

Evaluation of eye injury degree of laser irradiation frequency and single duration

Authors: Zhang Yufei,  Wei Dongyu,  Wang Wei,  Liu Daming,  Ren Ze,  Li Xiangqian,  Chen Tao,  Zhang Zuoming DOI: 10.3760/cma.j.cn115989-20200105-00007 Published 2021-03-10 Cite as Chin J Exp Ophthalmol, 2021, 39(3): 184-190. Abstract Objective To explore the effects of laser irradiation parameters (irradiation frequency and single duration) on tear secretion, lens and retina. Methods Thirty-six healthy guinea […]

Protective effect of vitexin on retinal ganglion cells in rat retinal ischemia-reperfusion injury model

Authors: Li Manli,  Fan Ke,  Cui Hongpei DOI: 10.3760/cma.j.cn115989-20200509-00319 Published 2021-03-10 Cite as Chin J Exp Ophthalmol, 2021, 39(3): 191-197. Abstract Objective To explore the protective effect of vitexin on retinal ganglion stem cells (RGCs) from oxidative stress caused by retinal ischemia-reperfusion (RIR) in rats and its possible mechanism. Methods Sixty male SD rats were […]

Repair effect of platelet-rich plasma on traumatic optic nerve injury in rabbits and its mechanism

Authors: Li Linling,  Xing Yiqiao DOI: 10.3760/cma.j.cn115989-20200701-00468 Published 2021-03-10 Cite as Chin J Exp Ophthalmol, 2021, 39(3): 198-206. Abstract Objective To study the therapeutic effect and mechanism of autologous platelet-rich plasma (PRP) on rabbit traumatic optic neuropathies (TON) and retina. Methods Forty adult New Zealand white rabbits were selected to establish the optic nerve clamp […]

Inhibitory effects of lncRNA ADPGK-AS1 on the biological behaviours of human retinoblastoma cells and its regulating mechanism

Authors: Zhang Jun,  Liu Cailin,  Bu Zhanyun DOI: 10.3760/cma.j.cn115989-20200819-00599 Published 2021-03-10 Cite as Chin J Exp Ophthalmol, 2021, 39(3): 207-215. Abstract Objective To explore the effects of long noncoding RNA adenosine diphosphate-dependent glucokinase antisense RNA 1(ADPGK-AS1) on the proliferation, migration and invasion of human retinoblastoma (RB) Y-79 cells and its regulatory effect on microRNA-623 (miR-623). […]

Protocol for the rapid quantitative analysis of neutrophils in mouse cornea by flow cytometry

Authors: Xue Yunxia,  Liu Jun,  Li Zhijie DOI: 10.3760/cma.j.cn115989-20200429-00297 Published 2021-02-10 Cite as Chin J Exp Ophthalmol, 2021,39(02): 102-106. Abstract Objective To provide a standard protocol for the rapid quantitative analysis of neutrophils in inflamed corneas with flow cytometry. Methods The corneal epithelium layer of 15 C57BL/6 mice (6-8 weeks old) was mechanically scraped off […]

Establishment and evaluation of a recurrent infection model of herpes simplex keratitis in BALB/c mice

Authors: Xiao Shuyu,  Yu Ying,  Tao Jinhua DOI: 10.3760/cma.j.cn115989-20191210-00535 Published 2021-02-10 Cite as Chin J Exp Ophthalmol, 2021,39(02): 107-112. Abstract Objective To explore the establishment and evaluation method of recurrent infection model of herpes simplex keratitis (HSK). Methods To determine the optimal duration of the ultraviolet light irradiation, 12 healthy BALB/c mice were randomized into […]

Biocompatibility of fibrin sealant and human corneal fibroblasts

Authors: Ye Qing,  Jiang Linzhi,  Chen Wenlin,  Zhang Wei,  Zeng Jing DOI: 10.3760/cma.j.cn115989-20190605-00244 Published 2021-02-10 Cite as Chin J Exp Ophthalmol, 2021,39(02): 113-118. Abstract Objective To study the biocompatibility of fibrin sealant (FS) and human corneal fibroblasts (HCFs) obtained by small incision lenticule extraction (SMILE). Methods The human corneal stromal tissues were selected from corneal […]

Comparison of fluorescence staining and periodic acid-Schiff staining in the diagnosis of fungal keratitis

Authors: Du Man,  Zhang Li,  Li Peng,  Qi Hongmei,  Lu Xiuhai DOI: 10.3760/cma.j.cn115989-20190702-00284 Published 2021-02-10 Cite as Chin J Exp Ophthalmol, 2021,39(02): 119-125. Abstract Objective To compare the effect of fluorescence staining and periodic acid-Schiff staining in the diagnosis of fungal keratitis (FK). Methods A total of 147 corneal specimens from 147 FK patients treated […]

Safety of intravitreal injection of recombinant human endostatin in rabbits

Authors: Xia Ying,  Chen Fang,  Du Wei,  Zhu Jun,  Jia Shuya,  Ma Haiying,  Xie Zhenggao DOI: 10.3760/cma.j.cn115989-20201104-00744 Published 2021-01-10 Cite as Chin J Exp Ophthalmol, 2021,39(01): 1-6. Abstract Objective To investigate and evaluate the safety of intravitreal injection of recombinant human endostatin (rh-endostatin) with different concentrations in rabbit eyes. Methods Thirty healthy adult New Zealand […]

Protective effect of Radix Astragali seu Hedysari compound on rat retinal DNA damage induced by acute hypobaric hypoxia

Authors: Li Yanrong,  Xin Xiaorong DOI: 10.3760/cma.j.cn115989-20190903-00380 Published 2021-01-10 Cite as Chin J Exp Ophthalmol, 2021,39(01): 7-12. Abstract Objective To investigate the rat retinal DNA damage induced by acute hypobaric hypoxia and the protective effect of Radix Astragali seu Hedysari compound on it. Methods Seventy-two healthy clean grade male Sprague Dawley (SD) rats were randomly […]