Category: Experimental Science

Effects of different intensity of lighting on refractive development and form deprivation myopia in guinea pigs

Authors: Li Congying,  Gan Jiahe,  Wang Meijun,  Cao Beihe,  Huang Ying,  He Xi,  Hua Ziyu,  Sun Minghao,  Li Shiming DOI: 10.3760/cma.j.cn115989-20220228-00079 Published 2022-06-10 Cite asChin J Exp Ophthalmol, 2022, 40(6): 491-497. Abstract Objective To investigate the effects of different intensity of lighting on normal refractive development and form deprivation myopia (FDM) in guinea pigs. Methods […]

Inhibitory effect of siRNA-Pax6 on biological behavior and epithelial-mesenchymal transition of human lens epithelial cells

Authors: Zheng Yuxing,  Yang Xiaoxi,  Yi Guoguo,  Wu Shuduan,  Feng Zhizhen,  Xia Zhaoxia DOI: 10.3760/cma.j.cn115989-20210423-00272 Published 2022-06-10 Cite asChin J Exp Ophthalmol, 2022, 40(6): 499-506. Abstract Objective To explore the effect of knockdown of the homeobox gene paired-box 6 (Pax6) on the biological behavior and epithelial-mesenchymal transition (EMT) of human lens epithelial cells (LECs). Methods […]

Inhibitory effects of NADPH oxidase 4 inhibitor on the epithelial-mesenchymal transition of human RPE cells induced by bevacizumab

Auhtors: Xie Chaohui,  Hao Xianghui,  Yang Lingling,  Xu Haifeng DOI: 10.3760/cma.j.cn115989-20210407-00234 Published 2022-06-10 Cite asChin J Exp Ophthalmol, 2022, 40(6): 507-513. Abstract Objective To observe the influence of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) 4 inhibitors on epithelial-mesenchymal transition (EMT) of human retinal pigment epithelial (RPE) cells induced by bevacizumab. Methods The cultured ARPE-19 […]

Inhibitory effect of siRNA-KCNQ1OT1 on human lens epithelial cell apoptosis by targeting miR-199a-5p

Authors: Lu Cheng,  Zhang Fengyan,  Zhang Yuhang,  Zhang Jiajuan DOI: 10.3760/cma.j.cn115989-20210427-00280 Published 2022-06-10 Cite asChin J Exp Ophthalmol, 2022, 40(6): 514-523. Abstract Objective To explore the inhibitory effect of long non-coding RNA (lncRNA) KCNQ1 overlapping transcript 1 (KCNQ1OT1) by targeting microRNA-199a-5p (miR-199a-5p) on the apoptosis of human lens epithelial cells (LECs). Methods The anterior lens […]

Inhibition of lens epithelial cell migration using different square-edge intraocular lenses in an in vitro capsular bag model

Authors: Lian Feiyue,  Li Yang,  Jiang Lingfeng,  Shen Haoyue,  Zhao Jiangyue,  Yan Xiaoxiao,  Yu Jiaming,  Qin Yu DOI: 10.3760/cma.j.cn115989-20200514-00346 Published 2022-05-10 Cite asChin J Exp Ophthalmol, 2022, 40(5): 395-402. Abstract Objective To establish an in vitro capsular bag model and compare the inhibitory effects of different 360° square-edge intraocular lens (IOL) on lens epithelial cells […]

Regulation of miR-155 in H2O2-induced oxidative stress in lens epithelial cells via targeting SIRT1

Authors: Niu Yantao,  Zhang Li,  Xie Wenfang DOI: 10.3760/cma.j.cn115989-20201104-00735 Published 2022-05-10 Cite asChin J Exp Ophthalmol, 2022, 40(5): 404-413. Abstract Objective To investigate the role of microRNA (miR)-155 in hydrogen peroxide (H2O2)-induced oxidative stress injury in lens epithelial cells (LECs) and its mechanism regulating silent information regulator factor related enzymes 1 (SIRT1). Methods The HLE-B3 […]

Inhibitory effect of melatonin on pyroptosis of lens epithelium cells and its mechanism

Authors: Yang Xin,  Liu Xuhui,  Meng Jia,  Fang Mengyuan,  Zhang Fengyan DOI: 10.3760/cma.j.cn115989-20210516-00307 Published 2022-05-10 Cite asChin J Exp Ophthalmol, 2022, 40(5): 414-421. Abstract Objective To investigate the inhibitory effect of melatonin on pyroptosis of lens epithelium cells (HLECs) induced by hydrogen peroxide (H2O2) and its mechanism. Methods The cultured HLECs were divided into normal […]

Promoting effect of miR-15a on high glucose-induced oxidative stress in human lens epithelial cells and its mechanism

Authors: Nan Anchao,  Nan Jianru,  Liu Yadong,  Liu Xiangling DOI: 10.3760/cma.j.cn115989-20200301-00126 Published 2022-05-10 Cite asChin J Exp Ophthalmol, 2022, 40(5): 422-430. Abstract Objective To investigate the effect of microRNA-15a (miR-15a) on the anti-oxidative stress ability of human lens epithelial cells (LECs) induced by high glucose and its possible mechanism. Methods The anterior lens capsule specimens […]

Specific expression of transmembrane protein 26 in retina and its association with primary open-angle glaucoma

Authors: Yin Yi,  Mao Yao,  Yang Zhenglin,  Huang Lulin DOI: 10.3760/cma.j.cn115989-20200112-00021 Published 2022-04-10 Cite asChin J Exp Ophthalmol, 2022, 40(4): 303-309. Abstract Objective To predict the transmembrane structure of transmembrane protein 26 (TMEM26), observe its expression in human retina and mouse retina, and investigate the relationship between it and primary open-angle glaucoma (POAG). Methods The […]

Inhibitory effect of necrostatin-1 on necroptosis of retinal ganglion cells in rats with acute ocular hypertension

Authors: Jing Yuanyuan,  Ma Yi,  Wang Kai,  Liu Zhuqing DOI: 10.3760/cma.j.cn115989-20191108-00488 Published 2022-04-10 Cite asChin J Exp Ophthalmol, 2022, 40(4): 310-315. Abstract Objective To investigate the inhibitory effect of specific inhibitor of necroptosis necrostatin-1 (Nec-1) on necroptosis of retinal ganglion cells (RGCs) in rats with acute ocular hypertension. Methods Twenty-four adult male Sprague Dawley rats […]