Current research and challenge of human retinal organoid differentiation

Authors: Luo Ziming,  Ge Jian
DOI: 10.3760/cma.j.cn115989-20200721-00518
Published 2020-10-10

Cite as Chin J Exp Ophthalmol, 2020,38(10): 809-812.

Abstract                              [View PDF] [Read Full Text]

The retinal organoid is one of the most widely studied organoids in vitro, which is an “organ in a dish” with a highly similar tissue structure to those in vivo.Since it was first reported, the differentiation methods of retinal organoids have been continously developed to improve the differentiation efficiency and achieve further maturation.Retinal organoids have a broad application prospect as developmental and disease models, as well as seed cells for replacement therapy.However, there are still some problems to be solved, including the universality, effectiveness, heterogeneity of retinal organoid differentiation, as well as the difference between retinal organoids development and the real fetal retinal development.Researchers should investigate the regulational mechanism during organoid development from molecular, genetic, cellular and histological structure levels in order to promote the clinical practices of organoid culture technology.

Key words:

Stem cells; Retinal organoid; Induced differentiation

Contributor Information

Luo Ziming
State Key Laboratory of Ophthalmology, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China; School of Medicine, Stanford University, Palo Alto, CA94304, USA
Ge Jian
State Key Laboratory of Ophthalmology, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China
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Updated: December 26, 2022 — 1:51 am