Category: 2020, No. 10

Current research and challenge of human retinal organoid differentiation

Authors: Luo Ziming,  Ge Jian DOI: 10.3760/cma.j.cn115989-20200721-00518 Published 2020-10-10 Cite as Chin J Exp Ophthalmol, 2020,38(10): 809-812. Abstract The retinal organoid is one of the most widely studied organoids in vitro, which is an “organ in a dish” with a highly similar tissue structure to those in vivo.Since it was first reported, the differentiation methods […]

Generation of VSX2 fluorescent reporter human induced pluripotent stem cells by CRISPR/Cas9 technology

Authors: Zheng Dandan,  Wang Yuan,  Zhang Zuming,  Guan Yuanyuan,  Xie Bingbing,  Jin Kangxin,  Xiang Mengqing,  Zhong Xiufeng DOI: 10.3760/cma.j.cn115989-20200729-00535 Published 2020-10-10 Cite as Chin J Exp Ophthalmol, 2020,38(10): 814-820. Abstract Objective To establish a fluorescent reporter human induced pluripotent stem cell line (hiPSCs) for monitoring the expression of visual system homeobox 2 (VSX2). Methods VSX2_small […]

Evaluation of the retinal differentiation ability of human induced pluripotent stem cells reprogrammed from different somatic cells

Authors: Xie Bingbing,  Guan Yuanyuan,  Gao Guanjie,  Zheng Dandan,  Xu Ping,  Song Xiaojing,  Zhong Xiufeng DOI: 10.3760/cma.j.cn115989-20200805-00563 Published 2020-10-10 Cite as Chin J Exp Ophthalmol, 2020,38(10): 821-827. Abstract Objective To evaluate the retinal differentiation ability of human induced pluripotent stem cells (hiPSCs) from various somatic cell sources. Methods The hiPSCs lines BC1- green fluorescent protein […]

Transplantation of retinal organoids in vivo and long-term survival of photoreceptor cells

Authors: Sun Xiao,  Xie Haohuan,  Zhang Mei,  Xue Tian DOI: 10.3760/cma.j.cn.115989-20200331-00227 Published 2020-10-10 Cite as Chin J Exp Ophthalmol, 2020,38(10): 829-836. Abstract Objective To investigate the differentiation process of human-induced pluripotent stem cells (hiPSCs) into retinal organoids (ROs) in vitro and its potential application in cell transplantation, and to provide a fundamental study for stem-cell […]

Differentially expressed genes analysis on the role of Wnt5a in lentoid body induction from human embryonic stem cells

Authors: Chen Yijia,  Li Jinyan,  Ouyang Shuai,  Luo Lixia DOI: 10.3760/cma.j.cn115989-20200308-00156 Published 2020-10-10 Cite as Chin J Exp Ophthalmol, 2020,38(10): 837-844. Abstract Objective To investigate the mechanism of Wnt5a in lentoid body (LB) induction from human embryonic stem cells (hESCs). Methods A “three-stage” protocol was used for LB differentiation from hESCs in vitro, and Wnt5a […]

Association between LIPC gene polymorphisms and choroidal neovascularization

Authors: Liao Ling,  Hao Fang,  Jiang Dan,  Huang Lulin,  Chen Haoyu,  Yang Zhenglin DOI: 10.3760/cma.j.cn115985-20190124-00030 Published 2020-10-10 Cite as Chin J Exp Ophthalmol, 2020,38(10): 845-850. Abstract Objective To investigate the association between choroidal neovascularization (CNV) and the LIPC gene single nucleotide polymorphism (SNP) in a Chinese Han population from Shantou. Methods A case-control study was […]

A comparison of postoperative efficacy and safety between laser-assisted subepithelial keratomileusis versus femtosecond laser assisted in situ keratomileusis for high myopic eyes

Authors: Liu Mingna,  Gao Hua,  Li Na,  Shi Weiyun DOI: 10.3760/cma.j.cn115989-20200818-00597 Published 2020-10-10 Cite as Chin J Exp Ophthalmol, 2020,38(10): 851-857. Abstract Objective To evaluate and compare the safety, efficacy, stability and predictability of laser-assisted subepithelial keratomileusis (LASEK) with femtosecond laser assisted in situ keratomileusis (FS-LASIK) for high myopic eyes. Methods A non-randomized controlled clinical […]

Corneal endothelial cell density and morphology of cataract eyes in Danzhou city Hainan province

Authors: Huang Jing,  Du Yali,  Zhang Mingzhi DOI: 10.3760/cma.j.cn115989-20200519-00353 Published 2020-10-10 Cite as Chin J Exp Ophthalmol, 2020,38(10): 858-863. Abstract Objective To describe corneal endothelial cell density and morphology in cataract eyes in Danzhou city, Hainan province and analyze the influencing factors. Methods A cross-sectional study was performed.A total of 573 eyes of 573 cataract […]

Analysis of National Natural Science Foundation of China grant support for basic research of ophthalmology during the past ten years

Authors: Wang Xiuhua,  Yang Yongguang,  Zhang Meng,  Lei Bo DOI: 10.3760/cma.j.cn115989-20200610-00416 Published 2020-10-10 Cite as Chin J Exp Ophthalmol, 2020,38(10): 864-870. Abstract Objective To review the detail data of National Natural Science Foundation of China (NSFC) grant support during past decade and to analyze the tendency of basic and applied science researches in ophthalmology and […]

Dry eye consensus by Asian Dry Eye Society: interpretation

Authors: Wang Hua,  Liu Zuguo DOI: 10.3760/cma.j.cn115989-20200828-00614 Published 2020-10-10 Cite as Chin J Exp Ophthalmol, 2020,38(10): 871-876. Abstract Asia Dry Eye Society (ADES) is the leading dry eye academic organization in Asia.Based on Dry Eye Work Shop (DEWS)Ⅰin 2007 and DEWSⅡin 2017 released by Tear Film and Ocular Surface Association (TFOS), ADES developed a systematic […]