Comparison of the capsule biocompatibility of three types of intraocular lenses in vitro

Authors:Huang Qi,  Zhao Jian,  Dang Yalong,  Zheng Bowen,  Hu Zhipeng,  Wang Guiqin
DOI: 10.3760/cma.j.issn.2095-0160.2016.05.005
Published 2016-05-10
Cite as Chin J Exp Ophthalmol, 2016,34(5): 402-407.

Abstract

Background

Researchers showed that posterior capsule opacification (PCO) may be associated with the materials of intraocular lenses (IOLs). However, there have long been controversies about the influence of IOLs materials on PCO pathogenesis.

Objective

This study was to compare the capsule biocompatibility of different materials of IOLs by observing the biological behavior of human lens epithelial cells (LECs) on the surface of IOLs, including adhesion, proliferation, epithelial mesenchymal transition (EMT) and the secretion of transforming growth factor-β2(TGF-β2), interleukin-6 (IL-6), matrix metalloproteinase-2 (MMP-2) and MMP-9 in vitro.

Methods

Human lens epithelial cell line (HLE-B3) was cultured on the surface of hydrophobic acrylic (Acrysof SA60AT) IOLs, silicone (Crystalens HD) IOLs and polymethyl methacrylate (PMMA) IOLs for 6 hours and 24 hours, respectively.The number and morphology of HLE-B3 cells on the surface of IOLs were observed under the optical microscope.The proliferation states of the cells on the IOL surface were detected with cell counting kit-8 (CCK-8). The expression of α-smooth muscle actin (α-SMA), a mesenchymal cell marker, in the cells was detected by immunofluorescence technology, and the EMT rates of HLE-B3 cells were calculated.The contents of TGF-β2, IL-6, MMP-2 and MMP-9 in the medium of IOL surface were measured by ELISA assay.The examination results were compared among different IOLs.

Results

Six hours after cultured, attached cells showed polygon and round in shape on the surface of Acrysof IOLs and Crystalens IOLs, while those on the PMMA IOLs showed the fusiform.Twenty-four hours after cultured, the cells extended obviously.On the surface of Acrysof IOLs and Crystalens IOLs, the adherent cells showed less cobble-stone like and more spindle shape; while those on the PMMA IOLs showed the typical fiber and some cells clustered the pearl rolls.The number of the cells on the Acrysof IOLs, Crystalens IOLs and PMMA IOLs was 0.238 4±0.007 1, 0.178 1±0.006 6 and 0.158 9±0.006 9 respectively, showing a significant difference among the three types of IOLs (F=475.947, P=0.000), and the number of the cells was much more on the Acrysof IOLs than that on the Crystalens IOLs or PMMA IOLs (both at P<0.001). The EMT rats of the cells on the Acrysof IOLs, Crystalens IOLs and PMMA IOLs were (9.99±3.80) %, (17.33±5.71) % and (84.16±10.48) %, with a significant difference among them (F=127.411, P=0.000), and the EMT rates of the cells on the PMMA IOLs were significantly higher than those on the Crystalens IOLs and Acrysof IOLs(both at P<0.001). There were significant differences in the contents of TGF-β2, IL-6, MMP-2 and MMP-9 in the medium on the Acrysof IOLs, Crystalens IOLs and PMMA IOLs (F=0.846, 0.947, 0.255, 0.922, all at P>0.05).

Conclusions

The hydrophobic acrylic IOLs have the best capsule biocompatibility followed by the silicone IOLs and then the PMMA IOLs.

Key words:

Biocompatible materials; Capsule opacification/etiology; Lenses, intraocular/adverse effects; Posterior capsule of the lens/pathology; Epithelial cells, lens; Human

Contributor Information

Huang Qi
Department of Ophthalmology, Third Clinical Medical College of Southern Medical University, Guangzhou 510515, China
Zhao Jian
Laboratory of Retina Brain Research, Department of Ophthalmology, The University of Hong Kong, Hong Kong SAR, Hong Kong 999077, China
Dang Yalong
Laboratory of Retina Brain Research, Department of Ophthalmology, The University of Hong Kong, Hong Kong SAR, Hong Kong 999077, China
Zheng Bowen
Hong Kong Laser Eye Center, Hong Kong 999077, China
Hu Zhipeng
Hong Kong Laser Eye Center, Hong Kong 999077, China
Wang Guiqin
Department of Ophthalmology, Third Clinical Medical College of Southern Medical University, Guangzhou 510515, China
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