Effects of groupⅠmetabotropic glutamate receptor on the synaptic transmission efficiency of the neurons in primary visual cortex of monocular form deprivated rats

Authors:Zhang Rui,  Liu Xiangling,  Lu Chengbiao,  Liu Zhihua,  Lin Jingjing,  Chen Yong,  Wang Yuanyue
DOI: 10.3760/cma.j.issn.2095-0160.2016.04.002
Published 2016-04-10
Cite as Chin J Exp Ophthalmol, 2016,34(4): 293-297.

Abstract

Background

The formation of amblyopia is closely associated with glutamate receptors of visual cortex, and researches determined that the expression of metabolic glutamate receptor 1 (mGluR1) in the visual cortex of amblyopic rats is down-regulated.However, the effect of mGluR1 on synaptic transmission of neurons in the visual cortex is unclear.

Objective

This study was to investigate the role of mGluR1 in the synaptic transmission efficiency of neurons in the primary visual cortex of monocular form-deprivated rats.

Methods

Sixteen 14-day-old SD rats were randomly divided into normal control group and form-deprivation group, with 8 rats in each group.The eyelids of the left eyes were sutured to establish the monocular form-deprivation models in the rats of the form-deprivation group, and the visual cortex slices were prepared until 31 days after modeling and incubated in artificial cerebrospinal fluid with 3, 5-dihydroxyphenylglycine (DHPG), LY367385+ DHPG, 2-methyl-6-(phenyl acetylene) pyridine hydrochloride(MPEP)+ DHPG and LY367385+ MPEP+ DHPG, respectively.The field excitatory postsynaptic potential (fEPSP) of neurons in the visual cortex of V1 area was detected by extracellular microelectrode recording.The use and care of the animals followed the statement for the Use of Animals in Ophthalmic and Vision Research.

Results

The slope value before drug treatment was set as 100%, the fEPSP-slopes of the normal control group and the form-deprivation group were increased to (136.4±17.3)% and (120.7±12.8)% after DHPG induction with significant difference between the two groups (t=2.280; P<0.05). The fEPSP-slopes of the normal control group were (114.9±9.3)% and (112.6±15.3)% after LY367385 and MPEP treatment, and those of the form-deprivation group were (107.3±6.0)% and (110.1±4.1)%, which were significantly lower than those after DHPG induction (normal control group: t=2.641, 2.915; both at P<0.05; form-deprivation group: t=2.410, 2.372; both at P<0.05). The fEPSP-slopes of the normal control group and the form-deprivation group were (104.5±2.2)% and (102.8±14.9)% after LY367385+ MPEP treatment, which were lower than that after DHPG induction (t=3.080, 2.306; both at P<0.05).

Conclusions

The effect of mGluR1 on the synaptic transmission of neurons in the visual cortex weakens after monocular form-deprivation in rats.Both mGluR1 and mGluR5 participate in the neuronic synaptic transmission on visual cortex area and appear to be equivalent contribution.

Key words:

Electrophysiology; Excitatory amino acid agonists/pharmacology; Excitatory amino acid antagonists/pharmacology; Phenylacetates/pharmacology; Receptors, metabotropic glutamate/physiology; Visual cortex/growth & development; Neuronic synaptic transmission/drug effect; Form deprivation; Rats, SD

Contributor Information

Zhang Rui
Department of Clinical Medicine, Xinxiang Medical University, Xinxiang 453003, China
Liu Xiangling
Department of Ophthalmology, Third Affiliated Hospital of Xinxiang Medical University, Xinxiang 453003, China
Lu Chengbiao
Laboratory of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang 453003, China
Liu Zhihua
Department of Clinical Medicine, Xinxiang Medical University, Xinxiang 453003, China
Lin Jingjing
Department of Ophthalmology, Xinyang Vocational Technical College Affiliated Hospital, Xinyang 464000, China
Chen Yong
Department of Clinical Medicine, Xinxiang Medical University, Xinxiang 453003, China
Wang Yuanyue
Department of Ophthalmology, Luohe City Central Hospital, Luohe 462000, China
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Updated: October 9, 2019 — 3:12 am