In vitro interactions between antifungals and tacrolimus against Fusarium solani

Authors: Chen Xiaolian,  Ding Hui,  Liu Hongshan,  He Hong,  Wu Jiaochan,  Hu Xianlyu,  Zhong Xingwu

DOI: 10.3760/cma.j.cn115989-20190613-00260
Published 2020-08-10
Cite as Chin J Exp Ophthalmol, 2020,38(08): 653-658.

Abstract

Objective

To investigate the in vitro interaction between antifungals and tacrolimus against Fusarium solani.

Methods

According to Clinical and Laboratory Standards Institute(CLSI) M27-Ed4 and M38-A3, 24 strains of Fusarium solani isolated from clinic were used to test the minimal inhibitory concentration (MIC) of the four antifungals.Chessboard microdilution method was used to evaluate the interaction of tacrolimus (FK506) combined with four antifungals in vitro.The study protocol was approved by the Ethics Committee of Hainan Eye Hospital (No.2017-006).

Results

The MIC ranges of fluconazole, voriconazole, natamycin and amphotericin B against 24 strains of Fusariumwere 64-256 μg/ml, 1-8 μg/ml, 2-8 μg/ml and 1-8 μg/ml, respectively.Tacrolimus combined with fluconazole, voriconazole, natamycin and amphotericin B had synergistic effects on 12.5% (3/24), 4.2% (1/24), 16.7% (4/24) and 29.2% (7/24) Fusarium, respectively.There were no antagonistic effect in this study.

Conclusions

Fusarium is sensitive to natamycin in vitro and partly sensitive to amphotericin B and voriconazole.Synergistic effects exist in few strains of Fusarium but no antagonistic effect.

Key words:

Antifungal agents; Fungal keratitis; Microbial sensitivity tests; Fusarium solani

Contributor Information

Chen Xiaolian
Hainan Eye Hospital and Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, SunYat-sen University, Haikou 570311, China
Ding Hui
Hainan Eye Hospital and Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, SunYat-sen University, Haikou 570311, China
Liu Hongshan
Hainan Eye Hospital and Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, SunYat-sen University, Haikou 570311, China
He Hong
Hainan Eye Hospital and Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, SunYat-sen University, Haikou 570311, China
Wu Jiaochan
Hainan Eye Hospital and Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, SunYat-sen University, Haikou 570311, China
Hu Xianlyu
Hainan Eye Hospital and Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, SunYat-sen University, Haikou 570311, China
Zhong Xingwu
Hainan Eye Hospital and Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, SunYat-sen University, Haikou 570311, China
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Updated: August 19, 2020 — 9:26 am