Induction of chorioretinopathy by adrenaline injection in different strains of rabbits

Authors: Yan Weiming,  Chen Tao,  Long Pan,  Ren Ze,  Li Xiangqian,  Sun Kai,  Ma Yifei,  Wang Xiaocheng,  Xue Junhui,  Zhang Zuoming

DOI: 10.3760/cma.j.issn.2095-0160.2018.01.005
Published 2018-01-10
Cite as Chin J Exp Ophthalmol, 2018,36(1): 23-27.

Abstract

Objective

To establish a central serous chorioretinopathy(CSC) model on different strains of rabbits by intravenous injection of adrenaline, which may contribute to related researches of CSC.

Methods

This study was approved by Bioethics Committee of Fourth Military University and complied with Statement for the Use of Animals in Ophthalmic and Visual Research.Fundus fluorescein angiography(FFA) was initially performed on male New Zealand white rabbits (10), Belgium rabbits (5) and Chinchilla rabbits(10) to make sure that the retinas of subjects were normal.For the New Zealand white rabbits, adrenaline was injected via ear vein at a dose of 0.04 mg/kg once per day for the first 8 weeks and followed by a dose of 0.08 mg/kg for the next 4 weeks, while 0.04 mg/kg adrenaline was injected in the same way for 8 weeks in the Belgium rabbits and Chinchilla rabbits.FFA was performed every week after injection of adrenaline to evaluate the fluorescence leakage in ocular fundus.New Zealand white rabbits were sacrificed in 4 (3 rabbits), 8 (3 rabbits) and 12 weeks (4 rabbits) after injection respectively, and Belgium rabbits and Chinchilla rabbits were sacrificed in the 8 weeks after injection.The eyeballs of the rabbits were enucleated to prepare the retinal sections for histopathological examination after hematoxylin-eosin staining.The results of FFA and retinal structure were compared among different strains of rabbits.

Results

No fluorescence leakage was found by FFA in ocular fundus, and the retinal structure was normal in all the 10 New Zealand white rabbits during the experiment.Fluorescence leakage was found by FFA in 2 Belgium rabbits at 1 week and 2 weeks after injection respectively, and retinal detachment and depigmentation of retinal pigment epithelium (RPE) with an enlarged intercellular space were shown by hematoxylin-eosin staining.For the Chinchilla rabbits, fluorescence leakages were found in 7 rabbits throughout the whole period of adrenaline administration.Circumscribed retinal detachment, depigmentation of RPE with enlarged intercellular space were also found in leakage lesions.

Conclusions

Repeated intravenous injection of adrenaline can induce CSC-like lesions in colored rabbits but not in albino rabbits.

Key words:

Adrenaline; Central serous chorioretinopathy; Disease model, animal/drug induction; Fundus fluorescein angiography; New Zealand rabbit; Belgium rabbit; Chinchilla rabbit

Contributor Information

Yan Weiming
Department of Clinical Medicine, Faculty of Aerospace Medicine, Key Laboratory of Aerospace Medicine of the National Education Ministry, Fourth Military University, Xi’an 710032, China
Chen Tao
Long Pan
Ren Ze
Li Xiangqian
Sun Kai
Ma Yifei
Wang Xiaocheng
Xue Junhui
Zhang Zuoming
(Read 51 times, 1 visits today)
Updated: September 4, 2019 — 7:26 am