Authors: Yan Weiming, Chen Tao, Long Pan, Ren Ze, Li Xiangqian, Sun Kai, Ma Yifei, Wang Xiaocheng, Xue Junhui, Zhang Zuoming
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To establish a central serous chorioretinopathy(CSC) model on different strains of rabbits by intravenous injection of adrenaline, which may contribute to related researches of CSC.
This study was approved by Bioethics Committee of Fourth Military University and complied with Statement for the Use of Animals in Ophthalmic and Visual Research.Fundus fluorescein angiography(FFA) was initially performed on male New Zealand white rabbits (10), Belgium rabbits (5) and Chinchilla rabbits(10) to make sure that the retinas of subjects were normal.For the New Zealand white rabbits, adrenaline was injected via ear vein at a dose of 0.04 mg/kg once per day for the first 8 weeks and followed by a dose of 0.08 mg/kg for the next 4 weeks, while 0.04 mg/kg adrenaline was injected in the same way for 8 weeks in the Belgium rabbits and Chinchilla rabbits.FFA was performed every week after injection of adrenaline to evaluate the fluorescence leakage in ocular fundus.New Zealand white rabbits were sacrificed in 4 (3 rabbits), 8 (3 rabbits) and 12 weeks (4 rabbits) after injection respectively, and Belgium rabbits and Chinchilla rabbits were sacrificed in the 8 weeks after injection.The eyeballs of the rabbits were enucleated to prepare the retinal sections for histopathological examination after hematoxylin-eosin staining.The results of FFA and retinal structure were compared among different strains of rabbits.
No fluorescence leakage was found by FFA in ocular fundus, and the retinal structure was normal in all the 10 New Zealand white rabbits during the experiment.Fluorescence leakage was found by FFA in 2 Belgium rabbits at 1 week and 2 weeks after injection respectively, and retinal detachment and depigmentation of retinal pigment epithelium (RPE) with an enlarged intercellular space were shown by hematoxylin-eosin staining.For the Chinchilla rabbits, fluorescence leakages were found in 7 rabbits throughout the whole period of adrenaline administration.Circumscribed retinal detachment, depigmentation of RPE with enlarged intercellular space were also found in leakage lesions.
Repeated intravenous injection of adrenaline can induce CSC-like lesions in colored rabbits but not in albino rabbits.