Research progress on oral mucosal epithelial culture and ocular surface transplantation

Authors: Gong Danni,  Yan Chenxi,  Fu Yao
DOI: 10.3760/cma.j.cn115989-20200304-00142
Published 2023-11-10
Cite as Chin J Exp Ophthalmol, 2023, 41(11): 1135-1139.

Abstract                           Download PDF】 【Read Full Text

Human corneal epithelial cells are continuously replenished by limbal stem cells to maintain ocular homeostasis and normal visual function.Some ocular surface injuries or diseases, such as thermochemical burns and Stevens-Johnson syndrome, can result in limbal stem cell deficiency, severely compromising patients’ visual acuity.Non-limbal autologous epithelial tissue transplantation should be considered when both eyes are affected.Oral mucosal epithelial cells are an important source of seed cells for ocular surface reconstruction.What’s more, cell sheets constructed ex vivo have achieved good results in clinical applications.This article reviews the research progress on the use of oral mucosal epithelial cells for the treatment of limbal stem cell deficiency, focusing on various factors including carriers, culture conditions, and techniques that may affect the culture of oral mucosal epithelial sheets.In addition, the strengths and weaknesses of the application of cell sheets in clinical ocular surface transplantation are also discussed, so as to provide a research direction for the safe and effective reconstruction of ocular surface epithelium.

Key words:

Oral mucosal epithelial cells; Culture in vitro; Limbal stem cell deficiency; Ocular surface reconstruction

Contributor Information

Gong Danni

Department of Ophthalmology, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai 200011, China

Yan Chenxi

Department of Ophthalmology, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai 200011, China

Fu Yao

Department of Ophthalmology, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai 200011, China

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