Application progress of flow cytometry in the cytokine test and cytological examination for ocular diseases

Authors: Liu Ming,  Tao Yong
DOI: 10.3760/cma.j.cn115989-20210118-00049 Published 2021-03-10
Cite as Chin J Exp Ophthalmol, 2021, 39(3): 269-272.

Abstract

As an advanced quantitative technology for cells, flow cytometry, which can make multi-paramter quantitative analysis and sorting of individual cells or biological particles, has been widely used in immunology, oncology, hematology and other fields of medical research and clinical diagnosis in recent years.It can quantitatively detect the expression levels of cytokines and the distribution characteristics of cell subsets in the intraocular fluid.Compared with traditional enzyme-linked immunosorbent assay, cell morphology and immunohistochemistry, it has the advantages of simple operation, smaller sample size, higher sensitivity and higher throughput.Flow cytometry has been widely used in the detection and subgroup analysis of cytokines related to eye diseases, such as intraocular angiogenesis, intraocular lymphoma, retinopathy, cataract, uveitis, sarcoidotic uveitis, infectious intraocular inflammation, etc.Flow cytometry plays an increasingly important role in the pathogenesis research and targeted therapy of eye diseases.In this article, the application of flow cytometry in the examination of cytokines and cytology in ocular fluid for ocular diseases were reviewed.

Key words:

Flow cytometry; Eye diseases; Intraocular fluid; Cytokine; Cell subsets

Contributor Information

Liu Ming
Beijing GiantMed Medical Diagnostics Laboratory, Beijing 101399, China
Tao Yong
Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
(Read 5 times, 1 visits today)
Updated: March 16, 2021 — 6:52 am