Comparision of the pharmacodynamics between different batches of recombinant decoy receptor innovative drug RC28-E in retinal vascular endothelial cells

Authors: Zheng Jialin,  Yang Qianhui,  Sun Jing,  Zhang Hong,  Jiang Jing,  Huang Min,  Li Shenjun,  Fang Jianmin,  Li Xiaorong,  Zhang Yan

DOI: 10.3760/cma.j.issn.2095-0160.2018.12.003
Published 2018-12-10
Cite as Chin J Exp Ophthalmol, 2018,36(12): 908-913.

Abstract

Objective

To compare the protective effects of pharmacological batch RC28-E1 and pilot batch RC28-E2 on retinal vascular endothelial cells (RF/6A) under the stimulation of vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF).

Methods

RF/6A cells were divided into normal control group, VEGF + FGF group and RC28-E1 groups with different concentrations.The optimal concentration of RC28-E1 was determined by cell counting kit-8 (CCK-8) method.Cells were divided into normal control group, VEGF+ FGF group, RC28-E1 group, RC28-E2 group, conbercept group and FGF trap group, and cultured with serum-free culture medium, serum-free culture medium containing VEGF+ FGF, serum-free culture medium containing VEGF+ FGF+ RC28-E1, serum-free culture medium containing VEGF+ FGF+ RC28-E2, and serum-free culture medium containing VEGF+ FGF+ conbercept, serum-free medium containing VEGF+ FGF+ FGF trap, respectively.Cell proliferation rate was measured by CCK-8 method, cell migration ability was detected by Transwell test, and tube formation ability was detected by Matrigel assay.

Results

The cell proliferation rate of 0.080 mg/ml RC28-E1 group was significantly lower than that of VEGF+ FGF group (P<0.05). The cell proliferation rate of RC28-E1 group, RC28-E2 group and FGF trap group were significantly lower than that of VEGF+ FGF group (P<0.05). The number of migrated cells in RC28-E1 group, RC28-E2 group, conbercept group and FGF trap group were significantly lower than that in VEGF+ FGF group (P=0.000). The numbers of meshes formed by retinal vascular endothelial cells in RC28-E1 group, RC28-E2 group, conbercept group and FGF trap group were significantly lower than that in VEGF+ FGF group (P=0.003, 0.001, 0.009, 0.018). The number of tube formation in FGF trap group was significantly higher than those in RC28-E1 group, RC28-E2 group, conbercept group and normal control group (P=0.014, 0.000, 0.008, 0.014).

Conclusions

Under the stimulation of VEGF+ FGF, the inhibitory effect of RC28-E on the proliferation of retinal vascular endothelial cells is greater than that of conbercept, and its inhibitory effect on the tube formation is superior to that of FGF trap.There is no significant difference in the effects of different batches of recombinant decoy receptor innovative drugs on retinal vascular endothelial cells.

Key words:

Proliferation; Migration; Tube formation; Angiogenesis; Recombinant decoy receptor

Contributor Information

Zheng Jialin
Tianjin Medical University Eye Hospital, Tianjin Medical University Eye Institute, College of Optometry and Ophthalmology, Tianjin Medical University, Tianjin 300384, China; School of Pharmacy, Binzhou Medical University, Binzhou 264003, China
Yang Qianhui
Tianjin Medical University Eye Hospital, Tianjin Medical University Eye Institute, College of Optometry and Ophthalmology, Tianjin Medical University, Tianjin 300384, China
Sun Jing
Tianjin Medical University Eye Hospital, Tianjin Medical University Eye Institute, College of Optometry and Ophthalmology, Tianjin Medical University, Tianjin 300384, China
Zhang Hong
Tianjin Medical University Eye Hospital, Tianjin Medical University Eye Institute, College of Optometry and Ophthalmology, Tianjin Medical University, Tianjin 300384, China
Jiang Jing
School of Pharmacy, Binzhou Medical University, Binzhou 264003, China
Huang Min
Remegen, Ltd., Yantai, 264006, China
Li Shenjun
Tianjin Medical University Eye Hospital, Tianjin Medical University Eye Institute, College of Optometry and Ophthalmology, Tianjin Medical University, Tianjin 300384, China
Fang Jianmin
School of Life Science and Technology, Tongji University, Shanghai 200092, China
Li Xiaorong
Zhang Yan
Tianjin Medical University Eye Hospital, Tianjin Medical University Eye Institute, College of Optometry and Ophthalmology, Tianjin Medical University, Tianjin 300384, China
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Updated: September 4, 2019 — 11:08 am