In vitroantimicrobial activity of diacerein on common ocular surface pathogens

Authors:Yue Juan,  Jian Shoujun,  Lyu Qixue,  Sun Shengtao,  Han Lei,  Liu Susu,  Zhang Hongmin

DOI: 10.3760/cma.j.issn.2095-0160.2020.03.002
Published 2020-03-10
Cite as Chin J Exp Ophthalmol, 2020,38(03): 165-168.

Abstract

Objective

To study the antimicrobial activity of diacerein on common pathogens of the ocular surface in vitro.

Methods

Pathogens were collected from patients with ocular surface infections in Henan Eye Hospital, including Gram-positive cocci and bacilli, Gram-negative bacilli, filamentous fungi, and Candida.The antimicrobial activity of diacerein was determined by the K-B agar diffusion method, and its minimum inhibitory concentration (MIC) was determined by the micro-liquid method.Levofloxacin and voriconazole were used as the control of antibacterial and antifungal drug, respectirely.

Results

Diacerein showed antibacterial activity against 42 strains of Gram-positive cocci and 10 strains of Gram-positive bacilli, its inhibition zone diameters for Staphylococcus epidermidis, S.aureus, S.intermedius and Gram-positive Corynebacterium were not significantly different from those of levofloxacin (all at P>0.05). Its MIC range of diacetate against Staphylococcus epidermidis, S. aureus, S. intermedius and other Staphylococci was 1-32 μg/ml, and its respective MIC90 was 16, 8, 16, and 32 μg/ml.Diacerein had no bacteriostatic effect on 23 strains of Gram-negative bacilli, 10 strains of filamentous fungi and 3 strains of candida.

Conclusions

Diacerein has antibacterial effects against Gram-positive Staphylococcus and Corynebacterium isolated from the ocular surface, but shows no antimicrobial activity against Gram-negative bacilli and fungi.Diacerein offers a new drug option and method for the treatment of bacterial keratitis.

Key words:

Gram-positive cocci; Bacilli; Filamentous fungi; Candida; Diacerein; Inhibition zone; K-B agar paper diffusion method

Contributor Information

Yue Juan
Department of Ophthalmology, Henan Provincial People’s Hospital, Henan Eye Hospital, Henan Eye Institute, Henan Key Laboratory of Ophthalmology and Visual Science, Zhengzhou University People’s eye Hospital, Zhengzhou 450003, China
Jian Shoujun
Lyu Qixue
Sun Shengtao
Han Lei
Liu Susu
Zhang Hongmin
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Updated: June 24, 2020 — 11:21 am