Category: 2019, No. 11

Focus on stem cell-based therapy for retinal degenerative diseases

Authors: Liu Qinghuai  DOI: 10.3760/cma.j.issn.2095-0160.2019.11.001 Published 2019-11-10 Cite as Chin J Exp Ophthalmol, 2019,37(11): 849-853. Abstract Retinal degenerative diseases are the common cause of blindness for all ages.Cell replacement therapy is the main strategy for treating retinal degenerative diseases.Recently, several retinal pigment epithelium(RPE) transplantation clinical trials have taken place worldwide and greatly enhanced the visual function […]

Regulation of human umbilical cord mesenchymal stem cells derived exosomes on peripheral blood macrophages from rabbit autoimmune dry eye

Authors: Li Na,  Nian Hong,  Zhao Lu,  Wei Yankai,  Wu Yiyuan,  Wei Ruihua DOI: 10.3760/cma.j.issn.2095-0160.2019.11.002 Published 2019-11-10 Cite as Chin J Exp Ophthalmol, 2019,37(11): 854-862. Abstract Objective To investigate the effect of human umbilical cord mesenchymal stem cells derived exosomes (hUC-MSC-exo) on the phenotype of peripheral blood macrophages from rabbit autoimmune dry eye and the expression […]

Protective effect of human umbilical cord mesenchymal stem cells on light-damaged retinal pigment epithelial cells

Authors: Zhuang Hong,  Zhang Rong,  Shu Qinmeng,  Zhang Shujie,  Xu Gezhi DOI: 10.3760/cma.j.issn.2095-0160.2019.11.003 Published 2019-11-10 Cite as Chin J Exp Ophthalmol, 2019,37(11): 863-869. Abstract Objective To investigate the protective effect of human umbilical cord mesenchymal stem cells (UCMSCs) on light-damaged retinal pigment epithelial (RPE) cells in vitro. Methods Human UCMSCs were cultured, and then identified by flow […]

Effects of shear stress on morphology, adhesion and proliferation of human umbilical cord blood mesenchymal stem cells

Authors: Duan Sujuan,  Cui Wenwen,  Liu Xing,  Huang Guofu DOI: 10.3760/cma.j.issn.2095-0160.2019.11.004 Published 2019-11-10 Cite as Chin J Exp Ophthalmol, 2019,37(11): 870-875. Abstract Objective To explore the effects of shear stress on morphology, adhesion and proliferation of human umbilical cord blood mesenchymal stem cells(hUC-MSCs). Methods The hUC-MSCs were cultured in vitro until confluence and then placed in a flow […]

The feasibility of PKH26-marked human adipose derived mesenchymal stem cells and intraocular trace

Authors: Guo Kai,  Luo Yan,  Li Shiqing,  Tian Jingyi DOI: 10.3760/cma.j.issn.2095-0160.2019.11.005 Published 2019-11-10 Cite as Chin J Exp Ophthalmol, 2019,37(11): 876-879. Abstract Objective To evaluate the feasibility of PKH26 marked human adipose derived mesenchymal stem cells (hADSCs) in vitro and intraocular. Methods HADSCs were cultured in vitro and marked with PKH26.Eighteen C57BL/6J mice were divided into normal control group (6 […]

The feasibility of human adipose-derived mesenchymal stem cells induced to endothelial-like cells and the safety of induced cells intraocular application in a short period

Authors: Guo Kai,  Luo Yan,  Li Shiqing,  Tian Jingyi DOI: 10.3760/cma.j.issn.2095-0160.2019.11.006 Published 2019-11-10 Cite as Chin J Exp Ophthalmol, 2019,37(11): 880-883. Abstract Objective To evaluate the feasibility of differentiation of human adipose mesenchymal stem cells (hADSCs) into endothelial-like cells and the safety of induced cells intraocular application. Methods HADSCs were induced to endothelial-like cells in vitro.The experimental […]

Appilcation of polycaprolactone/collagen nanofibrous membranes in constituting tissue engineering retinal pigment epithelium

Authors: Tang Tian,  Chen Jie,  Deng Hua,  Yin Xiaoguang,  Fu Yan DOI: 10.3760/cma.j.issn.2095-0160.2019.11.007 Published 2019-11-10 Cite as Chin J Exp Ophthalmol, 2019,37(11): 884-887. Abstract Objective To explore the potential of polycaprolactone (PCL)/gelatin nanofibrous composite membrane in constituting tissue engineering retinal pigment epithelium (RPE). Methods The embryonic stem cells (ESCs) were differentiated into RPE (ESC-RPE) cells by […]

Analysis of genotype and phenotype of hereditary retinal diseases which are easily misdiagnosed as amblyopia

Authors: Qi Rui,  Zhu Jinyan,  Wang Xiaoguang,  Zhuang Wenjuan,  Sheng Xunlun DOI: 10.3760/cma.j.issn.2095-0160.2019.11.008 Published 2019-11-10 Cite as Chin J Exp Ophthalmol, 2019,37(11): 888-895. Abstract Objective To analysis the genotype and phenotype of hereditary retinal diseases (HRD) which are easily misdiagnosed as amblyopia. Methods A case-control study was designed.The patients with HRD who were misdiagnosed as amblyopia […]

Genetic screening of the congenital aniridia and genotype-phenotype analysis

Authors: Li Jie,  Li Zhanrong,  Xing Yasi,  Peng Haiying,  Dai Shuzhen DOI: 10.3760/cma.j.issn.2095-0160.2019.11.009 Published 2019-11-10 Cite as Chin J Exp Ophthalmol, 2019,37(11): 896-900. Abstract Objective To explore the genotype-phenotype correlation among 3 pedigrees affected with congenital aniridia. Methods Clinical data and genomic DNA were collected and genetic variations were screened by whole-exome sequencing, with an emphasis […]

Analysis of anterior chamber morphological changes after small incision lenticule extraction

Authors: Zhang Yaohua,  Wang Yan DOI: 10.3760/cma.j.issn.2095-0160.2019.11.010 Published 2019-11-10 Cite as Chin J Exp Ophthalmol, 2019,37(11): 901-906. Abstract Objective To investigate the changes of anterior chamber profiles after small incision lenticule extraction(SMILE) and analyze the possible influencing factors. Methods A case observation study was designed.Thirty-one patients(59 eyes) with myopia and myopic astigmatism who underwent SMILE surgery […]