The reaction pattern rules of mouse retinal ganglion cell under different wavelengths of light stimulation

Objective

To explore the reaction pattern rules of mouse retinal ganglion cells potential under different wavelengths of light stimulation.

Methods

Thirty SPF grade 3-week-old C57BL/6 mice were used for ex vivo whole mount retina preparation.The cells firing activities were recorded on patch clamp system with on cell touch mode under stimulation of 400 nm, 580 nm and white light, respectively.According to different reactions to different light stimulation, the cells were classified into 400 nm sensitive RGC, 580 nm sensitive RGC and color vision insensitive RGC.Then the cells were further classified according to light ON type, light ON/OFF type or light OFF type.The RGC’s baseline firing pattern (baseline firing frequency, burst firing frequency) and light activation firing pattern (response pattern, light response firing frequency, light response firing amplification) were compared among different RGC classifications.

Results

Eighty-two RGCs were recorded in total.The frequency of spontaneous firing activity ranged from 0.00 Hz to 32.33 Hz among different RGCs.400 nm sensitive RGCs were 52(63.41%), 580 nm sensitive RGCs were 29(35.37%) and color vision insensitive RGC was 1(1.22%). OFF type RGC was the main cell type in 400 nm sensitive group (36.29%), and ON/OFF type RGC was the main cell type in 580 nm sensitive group (34.48%). The firing amplification in 580 nm sensitive RGC was (22.93±10.23)Hz, which was significantly higher than (14.44±10.11)Hz in 400 nm sensitive RGC (t=4.060, P=0.044). The firing amplification in 580 nm sensitive ON type RGC was (24.17±8.98)Hz, which was significantly higher than (11.12±10.35)Hz in 400 nm sensitive ON type RGC (t=5.373, P=0.021).

Conclusions

There is no specific firing pattern rules among different light sensitive RGCs.In the future, artificial color vision may be achieved through personalized electric stimulation and learning feedback strategy.