Category: 2019, No. 09

Focus on the treatment of inherited retinal diseases

Authors:Pang Jijing DOI: 10.3760/cma.j.issn.2095-0160.2019.09.001 Published 2019-09-10 Cite as Chin J Exp Ophthalmol, 2019,37(9): 689-693. Abstract Inherited retinal diseases (IRDs) are rare and incurable eye diseases.Gene therapy has become a new method for the treatment of IRDs.At present, at least 26 clinical trials of gene therapy involving at least 16 different IRDs genes are in progress or […]

Differentially expressed genes analysis of retinal vascular endothelial cells under hyperglycemia and hyperlipidemia induced by α-melanocyte-stimulating hormone

Authors:Wu Mianmian,  Guo Fang,  Li Yahong,  Liu Ke,  Zhang Yan DOI: 10.3760/cma.j.issn.2095-0160.2019.09.002 Published 2019-09-10 Cite as Chin J Exp Ophthalmol, 2019,37(9): 694-700. Abstract Objective To investigate the effect of α-melanocyte-stimulating hormone(α-MSH) on the expression of mRNA and long noncoding RNA (lncRNA) in retinal vascular endothelial cells stimulated by hyperglycemia and hyperlipidemia. Methods The simian retinal vascular […]

Analysis of differentially expressed genes of Staphylococcus aureus isolated from corneal ulcer and healthy conjunctival sac

Authors:Liu Qing,  Dong Yanling,  Huang Yusen DOI: 10.3760/cma.j.issn.2095-0160.2019.09.003 Published 2019-09-10 Cite as Chin J Exp Ophthalmol, 2019,37(9): 701-706. Abstract Objective To investigate the possible pathogenesis of Staphylococcus aureus (S.aureus) corneal ulcer by analyzing the differentially expressed genes (DEGs) of S.aureus isolated from the patients with corneal ulcer and healthy conjunctival sac. Methods Ten strains of S.aureus isolates were obtained from January to […]

Proteomic research of human retinal microvascular pericytes stimulated with high glucose

Authors:Xiao Mengran,  Zhang Xiaomin,  Shao Xianfeng,  Xiao Jing,  Yang Fuhua,  Zhang Hui,  Ea Vicki L,  Li Xiaorong DOI: 10.3760/cma.j.issn.2095-0160.2019.09.004 Published 2019-09-10 Cite as Chin J Exp Ophthalmol, 2019,37(9): 707-712. Abstract Objective To determine the changes of protein expressions in human retinal microvascular pericytes (HRMPCs) stimulated with high glucose by using quantitative proteomics, which provides new clues […]

Effect and potential signaling pathway of hedgehog protein on the function of human retinal microvascular endothelial cell

Authors:Li Yang,  Hu Qinrui,  Wang Bin,  Xia Huika,  Zhang Xiaohu DOI: 10.3760/cma.j.issn.2095-0160.2019.09.005 Published 2019-09-10 Cite as Chin J Exp Ophthalmol, 2019,37(9): 713-718. Abstract Objective To explore the impact of Hedgehog protein on human retinal microvascular endothelial cell(HRMEC)and its signaling pathway. Methods The cultured HRMECs were divided into normal control group, 0.5 μmol/L agonist group and 1.0 […]

Diagnosis of choroideremia in two Chinese families misdiagnosed as retinitis pigmentosa using next-generation sequencing

Authors:Wang Miaomiao,  Wang Zhuoshi,  Sun Yan,  Xia Yang,  He Wei DOI: 10.3760/cma.j.issn.2095-0160.2019.09.006 Published 2019-09-10 Cite as Chin J Exp Ophthalmol, 2019,37(9): 719-724. Abstract Objective To identify choroideremia and retinitis pigmentosa(RP)using next-generation sequencing(NGS)technology. Methods A cross-sectional study was adopted.The participants were two pedigrees that was suspected of RP in previous hospital treatments in He Eye Specialist Hospital […]

Mutations analysis of FRMD7 gene in idiopathic congenital nystagmus families

Authors:Du Wei,  Zhang Ye,  Xie Zhenggao DOI: 10.3760/cma.j.issn.2095-0160.2019.09.008 Published 2019-09-10 Cite as Chin J Exp Ophthalmol, 2019,37(9): 726-729. Abstract Objective To reveal the pathogenic mutations in Chinese families with idiopathic congenital nystagmus(ICN) Methods Six families with ICN were recruited from Subei People’s Hospital.DNA was extracted from peripheral blood samples of all participants.All coding and exon-intronic boundary […]

Clinical manifestation and gene mutation of Bietti crystalline corneoretinal dystrophy

Authors:Guo Tong,  Jia Ruixuan,  Chen Ningning,  Yang Liping DOI: 10.3760/cma.j.issn.2095-0160.2019.09.009 Published 2019-09-10 Cite as Chin J Exp Ophthalmol, 2019,37(9): 730-735. Abstract Objective To analyze the clinical manifestation and CYP4V2 mutations of Bietti crystalline corneoretinal dystrophy( BCD) families. Methods Total of 234 patients (173 families) diagnosed as BCD were recruited in Peking University Third Hospital from 2010 to 2018.All […]

Mutation screen of P4HA2 gene in Tujia high myopia patients

Authors:Yang Lin,  Li Tuo,  Cai Xiaojun,  Ke Min,  Chen Zhongshan DOI: 10.3760/cma.j.issn.2095-0160.2019.09.010 Published 2019-09-10 Cite as Chin J Exp Ophthalmol, 2019,37(9): 736-739. Abstract Objective To investigate the mutation of P4HA2 gene in Tujia high myopia patients. Methods Clinical data and genomic DNA were collected from 288 Tujia patients with high myopia, whose spherical error ≥-6.00 diopters and axial […]

Screening of pathogenic mutation in a Chinese family with congenital pulverulent cataract

Authors:Ji Kangkang,  Gu Zhengyu,  Wang Yaru,  Bao Weili,  Liao Rongfeng DOI: 10.3760/cma.j.issn.2095-0160.2019.09.011 Published 2019-09-10 Cite as Chin J Exp Ophthalmol, 2019,37(9): 740-744. Abstract Objective To analysis the pathogenic mutation and the clinical characteristics of a three generation family with congenital pulverulent cataract. Methods A congenital cataract family was chosen from the First Affiliated Hospital of AnHui […]